Metastasis is the key result in of death in individuals with melanoma. Cannabis-primarily based medicines are productive adjunctive drugs in cancer patients. Tau and Stathmin proteins are the important proteins in cancer metastasis. Right here we have investigated the impact of a standardized Cannabis sativa extract on cell migration and Tau and Stathmin gene expression in the melanoma cell line.
Components AND Techniques:
In the remedy group, melanoma (B1617) was treated 48 hr with different concentrations of standardized C. sativa extract. Cells with no remedy had been viewed as as the handle group, then study was followed by Quantitative RT-Genuine Time PCR assay. Relative gene expression was calculated by the ΔΔct strategy. Migration assay was utilised to evaluate cancer metastasis.
Tau and stathmin gene expression was considerably decreased compared to the handle group. Cell migration was also considerably lowered compared to controls.
C. sativa decreased tau and stathmin gene expression and cancer metastasis. The outcomes may perhaps have some clinical relevance for the use of cannabis-primarily based medicines in individuals with metastatic melanoma.
Copyright © 2018 Elsevier B.V. All rights reserved.
PMID: 29147495 PMCID: PMC5673704 DOI: 10.22038/IJBMS.2017.9398
Vaseghi G1, Taki MJtwo, Javanmard SHtwo.